INVESTIGADORES
QUIROGA Evelina
congresos y reuniones científicas
Título:
Síntesis enzimática de péptidos en diferentes medios orgánicos
Autor/es:
EVELINA QUIROGA; JOSÉ MARCHESE; SONIA BARBERIS.
Lugar:
Sociedad de Biología de Cuyo
Reunión:
Congreso; XX Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2002
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
In this work we selected different organic media for the enzymatic synthesis of Z-Phe-Phe.OMe peptide (intermediary of a viral replication inhibitor, Z-Phe-Phe-Gly), using araujiain proteases. Hexane or NN-DMF and buffer (pH 8) or ethylene glycol (aw: 0.5 in the enzyme) were used as a reaction media. Reaction media were selected because of they were the most stabilizer for the enzyme, whereas ethylene glycol was selected because in it the enzyme showed the greater esterolytic activity/hydrolytic activity ratios. In hexano-buffer, the enzyme was able to produce the wished peptide. It is probable that the synthesis in NN-DMF (50 %) and in etylene glycol (aw: 0.5) has not taken place due to hydrolysis of the peptide, to viscosity of ethylene glycol (affecting the diffusion of the substrates towards the active site), to the presence of an unable nucleophile (probably one derivate more strongly activated will be required), and changes that could have undergone the enzyme (preventing the union of nucleophile to the intermediary acyl-enzyme).
