Correlation between the volume of vaginal cervical fluid and the growth of Tritrichomonas fetus.

MORERO, MARIANA; RAMIREZ M. ROSANA; PARADISO LANGHOFF FRANCO SERENO BRUNO, ESTEFANIA; ; OYHENART JORGE

virtual

Congreso; Reunion anual de sociedades de biociencias; 2020

Bovine trichomonosis is a venereal disease caused by Tritrichomonas fetus. The protozoan can be isolated from the cervical mucosa 2-3 months after intercourse and can reach the uterine mucosa where it is presumed to cause abortion. Cell growth dynamics, vaginal environmental factors, and pathogenicity mechanisms are not fully understood at this time. The objective of this study was to characterize the growth of T. fetus in cervical vaginal fluid (FCV) corresponding to different stages of the bovine estrous cycle in vitro. FCV was extracted from 3 animals with synchronized heat every 3 days for 3 successive estrous cycles. T. fetus was inoculated into FCV aliquots of different stages and growth was evaluated. The growth of the microorganism was clearly higher in oestrous fluids, also observing a stationary behavior and correlated with the volume of FCV extracted. The increased secretion of fluid in the estrous stage and its evident association with the proliferation of T. fetus allows us to understand the cell growth dynamics that the parasite could develop in vivo. A heat synchronization with a reproduction kit was performed on three female Alberdeen Angus breed bovines. Subsequently, the FCV was extracted every 3 days, with 10 mL syringes and 6 mm diameter silicone hoses, previously sterilized. After the collection of samples and once aliquoted in 1.5 mL tubes in volumes of 400 uL, they were inoculated with a discharge of 100,000 cells of T. fetus (B1) each, previously washed in NaCl (150 mM). They were then stored at 37ºC and counted at 24 and 48 hours, with "Neubauer Chambers". As can be seen in graph 1, the cross-correlogram indicates a significant correlation between the two variables studied: fluid volume and parasite growth. This is manifested with the correlation value at Lag 0 (0.8), exceeding the significance limit of 0.5, suggesting that an increase in the production volume of FCV (estrous stage) is associated with an increase in the number of T. fetus cells.