CULTIVATION OF TRITRICHOMONAS FOETUS IN BOVINE CERVICAL FLUID.

OYHENART, JORGE ANIBAL; MORERO, MARIANA; PARADISO LANGHOFF, FRANCO; RAMIREZ, ROSANA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencia 2019; 2019

SAIC

Abstract/Resumen: Tritrichomonas foetus is a flagellatedprotozoan bovine parasite. Although it can colonize other speciesthere are no known intermediate hosts, forms of free life orresistance. T. foetus is the causative agent of sexuallytransmitted disease known as bovine trichomoniasis (TB). TB iscommon in countries where extensive bovine breeding andnatural service are practiced, causing losses in calf production. Inthe bull the presence of T. foetus is asymptomatic while in thefemale it is recognized for a prolonged period between services,early abortions, vaginitis, among other aspects. In this study, theobjectives were in principle the synchronization and subsequentextraction of vaginal cervical fluid (FCV) belonging to thedifferent stages of the bovine estrous cycle; subsequently theculture of T. foetus cells in the aforementioned flow samples withthe purpose of finding differences that allow us to identify an?optimal culture moment?, if this exists. After the analysis of thedata, it was possible to observe that the FCV allows cell growth,counted at 48 hours, and does so differentially according to thestage of the estrous cycle to which the sample corresponds.Based on these results, it is possible to conclude that theproposed model is appropriate for the initially proposedobjectives