INCITAP 20787
INSTITUTO DE CIENCIAS DE LA TIERRA Y AMBIENTALES DE LA PAMPA
Unidad Ejecutora - UE
artículos
Título:
Loop mediated isothermal amplification method based on 5.8S ribosomal DNA sequences for specific detection of Tritrichomonas foetus
Autor/es:
OYHENART J, GRACIA MARTÍNEZ F, RAMIREZ R, BRECCIA J.
Revista:
VETERINARY PARASITOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 p. 59 - 65
ISSN:
0304-4017
Resumen:
Revealing the presence of Tritrichomonas foetus in bovine smegma is the basis of trichomonosis control. Loop-mediated isothermal amplification (LAMP) is a technique that rapidly amplifies a target DNA at steady temperature. A LAMP test was designed for a fast and accurate identification of T. foetus by targeting the 5.8S ribosomal DNA gene. The test was positive with 28 T. foetus isolates and negative with 36 non-T. foetus trichomonads assayed. Sensitivity with purified DNA, parasites in culture or spiked smegma showed results comparable to that obtained through PCR amplification with primers TFR3-TFR4. The detection limit was estimated around 4 x 103 cell/ml. The reproducibility of the LAMP assay using water bath and the ease in results readout show great potential for the diagnosis of T. foetus in rural areas.
