Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon

MARTIN VICTORIA; VILLARREAL FERNANDO; MIRAS ISABELLE; NAVAZA ALDA; HAOUZ AHMED; GONZÁLEZ-LEBRERO RODOLFO; KAUFMAN SERGIO; ZABALETA EDUARDO

FEBS LETTERS

ELSEVIER SCIENCE BV

Lugar: Alemania; Año: 2009 vol. 583 p. 3425 - 3430

0014-5793

Gamma carbonic anhydrases (cCA) are widespread in Prokaryotes. In Eukaryotes, homologous genes were found only in plant genomes. In Arabidopsis and maize, the corresponding gene products are subunits of mitochondrial Complex I.At present, only cCA homotrimers of Methanosarcina thermophila (CAM) show reversible carbon dioxide (CO2) hydration activity. In the present work, it is shown that recombinant plant Gamma -CA2 could form homotrimers and bind H14CO-3 . However, they are unable to catalyse the reversible hydration of CO2. These results suggest that plant cCAs do not act as carbonic anhydrases but with a related activity possibly contributing to recycle CO2 in the context of photorespiration.