Preparation, Characterization, and In Vitro Testing of Nanoclay Antimicrobial Activities and Elicitor Capacity

MANSILLA, ANDREA Y.; MERINO, DANILA; ALVAREZ, VERA A.; CASALONGUÉ, CLAUDIA A.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Año: 2018 vol. 66 p. 3101 - 3109

0021-8561

Clay-based nanocomposites (nanoclays) are interesting systems to hold a wide type of active substances with awide field of industrial applications. Bentonite−chitosan nanoclay was obtained via cationic exchange of natural bentonite (Bent)with an aqueous solution of chitosan (CS). Their physicochemical and morphological properties were discussed under the lightof Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and scanning electron microscopy.Bent−CS characterization indicated that CS was intercalated in 10% (w/w). This polycationic polymer was oriented mostly in amonolayer arrangement, interacting by electrostatic forces between Bent sheets. The antimicrobial action of Bent−CS nanoclaywas assayed onto phytopathogens, the bacterium model Pseudomonas syringe pv. tomato DC3000 (Psy) and the necrotrophicfungus Fusarium solani f. sp. eumartii (F. eumartii). In addition to demonstrating cell death on both microorganisms, Bent−CSexerted elicitor property on tomato plantlets. The biological actions of this natural nanomaterial might make it proper to be usedin crops.