IIB 20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Transgenic expression of plant-specific insert of potato aspartic proteases (StAP-PSI) confers enhanced resistance to Botrytis cinerea in Arabidopsis thaliana
Autor/es:
D IPPÓLITO, SEBASTIAN; FREY MARIA EUGENIA; PEPE ALFONSO; DALEO, GUSTAVO RAUL; GUEVARA MARIA GABRIELA
Revista:
PHYTOCHEMISTRY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2018 vol. 149 p. 1 - 11
ISSN:
0031-9422
Resumen:
Plant specific insert of Solanum tuberosum aspartic proteases (StAP-PSI) has high structural similarity with NK-lysin and granulysin, two saposin like proteins (SAPLIPS) with antimicrobial activity. Recombinant StAP-PSI, as well as some SAPLIPs, has antimicrobial activity on human and plant pathogens. In this work, we transformed A. thaliana plants with StAP-PSI encoding sequence with its corresponding signal peptide, under control of the CaMV 35S promoter. Results obtained show that StAP-PSI enhances significantly Arabidopsis resistance against B. cinerea infection. StAP-PSI is secreted into the leaf apoplast and acts directly against pathogens and therefore complement plant innate immune responses. Data obtained from real time PCR assays show that StAP-PSI constitutively expressed induces the expression of genes that regulate jasmonic acid signalling pathway, like PDF1.2, in response to necrotrophic pathogen infection. On the other hand and according with data described to others antimicrobial peptides, presence of StAP-PSI protein into the apoplast of A. thaliana leaves is responsible of the expression of salicylic acid associated gen, as PR-1, after or without infection with B. cinerea. These results indicate that the increased resistance of A. thaliana plants constitutively expressing StAP- PSI to B. cinerea respect to the wild- type plants is a consequence of two factors: 1- the antifungal activity of StAP-PSI and 2- the overexpression of A. thaliana defense genes induced by StAP-PSI constitutively expression. We suggest that the use of this protein would contribute to minimize the ecological and health risk relating with spreading resistances of agriculturally important pathogens.
