Immune modulation promoted by probiotic fermented milk in the mucosal immune system in an experimental model of allergy

VELEZ, EVA MARÍA; C. MALDONADO GALDEANO; CARMUEGA, ESTEBAN; WEILL, RICARDO; GABRIELA PERDIGÓN

Workshop; 6th International Immunonutrition Workshop.; 2013

Intestinal microbiota has an essential role in the maturation and function of the immune system. Changes in the microbiota, together with genetic factors induce the development of allergy with a typical Th2 profile(1). Several studies have shown that probiotics have a beneficial effect in disease and can stimulate the mucosal immune system by improving microbiota composition(2); hence, probiotics oral administration is proposed to avoid the allergy development by balancing microbiota and modulating the immune system, not only at the intestinal mucosa, but also in other mucosal sites and at systemic level(3). This study was aimed to evaluate the adjuvant immune effect of the oral administration of a probiotic fermented milk (PFM) in the intestinal mucosa and the extent of these effects on distant mucosal sites (bronchus) in an experimental mouse model of allergic airway reactivity to ovoalbumin (OVA). Experimental groups: normal-control (NC), Basal (B-5days-PFM); OVA-Sensitization-control (SC), Previous (P) (5d-PFM +OVA+ H2O) and Continuous (C) (5d- PFM +OVA+ PFM). SC, P and C were sensitized with OVA 1% followed by daily exposures to OVA aerosols. At 7 and 15 days postsensitization (dPS) specific-IgE, specific-IgG and IL-10 in serum, total S-IgA, IL-10 and IFN-g in intestinal fluid (IF), specific-IgE in bronchoalveolar lavage (BAL) were determined by ELISA. The number of IgA + , IL-10 + and IL-4 + cells in the lamina propria of small intestine (SI) and lungs were determined by immunofluorescense assay. In large intestine (LI) total populations of total anaerobes, lactobacilli, bifidobacteria and enterobacteria in selective media were determined. Specific-IgE was reduced in treated groups with regard to SC in serum and BAL (DO 450nm) (Table 1). Specific-IgG increased in all groups but showed the highest values in C