INV SUPERIOR JUBILADO
PERDIGON Gabriela Del Valle
artículos
Título:
Role of viability of probiotic strains in their persistence in the gut and in mucosal
Autor/es:
MALDONADO GALDEANO C; PERDIGÓN G
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Referencias:
Año: 2004 vol. 97 p. 673 - 681
ISSN:
1364-5072
Resumen:
Aims: To determine how probiotic bacteria contact with intestinal epithelial and immune cells and the conditions
to induce a good mucosal immune stimulation.To determine how probiotic bacteria contact with intestinal epithelial and immune cells and the conditions
to induce a good mucosal immune stimulation.
Methods and Results: Lactobacillus casei was studied by transmission electron microscopy (TEM) to determine
its interaction with the gut. We compared the influence of viable and nonviable lactic acid bacteria on the
intestinal mucosal immune system (IMIS) and their persistence in the gut of mice. TEM showed whole Lact.
casei adhered to the villi; the bacterial antigen was found in the cytoplasm of the enterocytes. Viable bacteria
stimulated the IMIS to a greater extent than nonviable bacteria with the exception of Lact. delbrueckii subsp.Lactobacillus casei was studied by transmission electron microscopy (TEM) to determine
its interaction with the gut. We compared the influence of viable and nonviable lactic acid bacteria on the
intestinal mucosal immune system (IMIS) and their persistence in the gut of mice. TEM showed whole Lact.
casei adhered to the villi; the bacterial antigen was found in the cytoplasm of the enterocytes. Viable bacteria
stimulated the IMIS to a greater extent than nonviable bacteria with the exception of Lact. delbrueckii subsp.Lact.
casei adhered to the villi; the bacterial antigen was found in the cytoplasm of the enterocytes. Viable bacteria
stimulated the IMIS to a greater extent than nonviable bacteria with the exception of Lact. delbrueckii subsp.adhered to the villi; the bacterial antigen was found in the cytoplasm of the enterocytes. Viable bacteria
stimulated the IMIS to a greater extent than nonviable bacteria with the exception of Lact. delbrueckii subsp.Lact. delbrueckii subsp.
bulgaricus. For all the strains assayed at 72 h no antigenic particles were found in the intestine.. For all the strains assayed at 72 h no antigenic particles were found in the intestine.
Conclusion: Antigenic particles but not the whole bacteria can enter to epithelial cells and contact with the
immune cells. Bacterial viability is a condition for a better stimulation of the IMIS.Antigenic particles but not the whole bacteria can enter to epithelial cells and contact with the
immune cells. Bacterial viability is a condition for a better stimulation of the IMIS.
Significance and Impact of the Study: We demonstrated that only antigenic particle interact with the immune
cells and their fast clearance from the gut agrees with those described for the particulate antigens. The regular
consumption of probiotics should not adversely affect the host.We demonstrated that only antigenic particle interact with the immune
cells and their fast clearance from the gut agrees with those described for the particulate antigens. The regular
consumption of probiotics should not adversely affect the host.