INV SUPERIOR JUBILADO
PERDIGON Gabriela Del Valle
artículos
Título:
Effects of milk fermented by Lactobacillus helveticus R389 on a murine breast cancer model
Autor/es:
DE MORENO DE LEBLANC, ALEJANDRA; MATAR, CHANTAL; LEBLANC, NICOLE; PERDIGON, GABRIELA
Revista:
Breast Cancer Research
Referencias:
Año: 2005 vol. 7 p. 477 - 486
ISSN:
1465-5411
Resumen:
Introduction Antitumour activity is one of the health-promoting
effects attributed to the lactic acid bacteria and their products
of fermentation. Previous studies in mice demonstrated that
bioactive compounds released in milk fermented byAntitumour activity is one of the health-promoting
effects attributed to the lactic acid bacteria and their products
of fermentation. Previous studies in mice demonstrated that
bioactive compounds released in milk fermented by
Lactobacillus helveticus R389 contribute to its
immunoenhancing and antitumour properties. The aim of the
present work was to study the effects of the consumption of milk
fermented by L. helveticus R389 or its proteolytic-deficient
variant, L. helveticus L89, on a murine hormone-dependent
breast cancer model.R389 contribute to its
immunoenhancing and antitumour properties. The aim of the
present work was to study the effects of the consumption of milk
fermented by L. helveticus R389 or its proteolytic-deficient
variant, L. helveticus L89, on a murine hormone-dependent
breast cancer model.L. helveticus R389 or its proteolytic-deficient
variant, L. helveticus L89, on a murine hormone-dependent
breast cancer model.L. helveticus L89, on a murine hormone-dependent
breast cancer model.
Methods Mice were fed with milk fermented by L. helveticusMice were fed with milk fermented by L. helveticus
R389 or L. helveticus L89, during 2 or 7 days. The tumour
control group received no special feeding. At the end of the
feeding period, the mice were challenged by a subcutaneous
injection of tumour cells in the mammary gland. Four days postinjection,
the mice received fermented milk on a cyclical basis.
The rate of tumour development and the cytokines in serum,
mammary gland tissue and tumour-isolated cells were
monitored. Bcl-2-positive cells in mammary glands and cellular
apoptosis in tumour tissue were also studied.L. helveticus L89, during 2 or 7 days. The tumour
control group received no special feeding. At the end of the
feeding period, the mice were challenged by a subcutaneous
injection of tumour cells in the mammary gland. Four days postinjection,
the mice received fermented milk on a cyclical basis.
The rate of tumour development and the cytokines in serum,
mammary gland tissue and tumour-isolated cells were
monitored. Bcl-2-positive cells in mammary glands and cellular
apoptosis in tumour tissue were also studied.
Results Seven days of cyclical administration of milk fermented
by either bacterial strain delayed or stopped the tumour
development. Cytokines demonstrated that L. helveticus R389
modulated the immune response challenged by the tumour. IL-
10 and IL-4 were increased in all the samples from this group. In
comparison with the tumour control, all test groups showed a
decrease of IL-6, a cytokine involved in oestrogen synthesis.
Seven days of cyclical feeding with milk fermented by L.
helveticus R389 produced an increase in the number of
apoptotic cells, compared with all other groups.Seven days of cyclical administration of milk fermented
by either bacterial strain delayed or stopped the tumour
development. Cytokines demonstrated that L. helveticus R389
modulated the immune response challenged by the tumour. IL-
10 and IL-4 were increased in all the samples from this group. In
comparison with the tumour control, all test groups showed a
decrease of IL-6, a cytokine involved in oestrogen synthesis.
Seven days of cyclical feeding with milk fermented by L.
helveticus R389 produced an increase in the number of
apoptotic cells, compared with all other groups.L. helveticus R389
modulated the immune response challenged by the tumour. IL-
10 and IL-4 were increased in all the samples from this group. In
comparison with the tumour control, all test groups showed a
decrease of IL-6, a cytokine involved in oestrogen synthesis.
Seven days of cyclical feeding with milk fermented by L.
helveticus R389 produced an increase in the number of
apoptotic cells, compared with all other groups.L.
helveticus R389 produced an increase in the number of
apoptotic cells, compared with all other groups.R389 produced an increase in the number of
apoptotic cells, compared with all other groups.
Conclusion This study demonstrated that 7 days of cyclical
administration of milk fermented by both strains of L. helveticusThis study demonstrated that 7 days of cyclical
administration of milk fermented by both strains of L. helveticusL. helveticus
diminishes tumour growth, stimulating an antitumour immune
response. Compounds released during milk fermentation with L.
helveticus R389 would be implicated in its immunoregulatory
capacity on the immune response in mammary glands and
tumour, which were correlated with the cytokines found at the
systemic level. The milk fermented by L. helveticus R389 was
able to modulate the relationship between immune and
endocrine systems (by IL-6 diminution), which is very important
in oestrogen-dependent tumour and induced cellular apoptosis.L.
helveticus R389 would be implicated in its immunoregulatory
capacity on the immune response in mammary glands and
tumour, which were correlated with the cytokines found at the
systemic level. The milk fermented by L. helveticus R389 was
able to modulate the relationship between immune and
endocrine systems (by IL-6 diminution), which is very important
in oestrogen-dependent tumour and induced cellular apoptosis.R389 would be implicated in its immunoregulatory
capacity on the immune response in mammary glands and
tumour, which were correlated with the cytokines found at the
systemic level. The milk fermented by L. helveticus R389 was
able to modulate the relationship between immune and
endocrine systems (by IL-6 diminution), which is very important
in oestrogen-dependent tumour and induced cellular apoptosis.L. helveticus R389 was
able to modulate the relationship between immune and
endocrine systems (by IL-6 diminution), which is very important
in oestrogen-dependent tumour and induced cellular apoptosis.