INV SUPERIOR JUBILADO
PERDIGON Gabriela Del Valle
artículos
Título:
Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-sever protein-energy-malnutrition model in mice.
Autor/es:
MALDONADO GALDEANO, CAROLINA; NOVOTNY NUñEZ IVANNA; DE MORENO DE LEBLANC, ALEJANDRA; CARMUEGA, ESTEBAN; WEILL, RICARDO; PERDIGON, GABRIELA
Revista:
BMC GASTROENTEROLOGY
Editorial:
BIOMED CENTRAL LTD
Referencias:
Año: 2011 vol. 11 p. 64 - 70
ISSN:
1471-230X
Resumen:
Malnutrition affects the immune response, causing a decrease of defence
mechanisms and making the host more susceptible to infections. Probiotics can reconstitute
the intestinal mucosa and stimulate local and systemic immunity. The aim of this work was
evaluate the effects of a probiotic fermented milk as a complement of a re-nutrition diet, on
the recovery of the intestinal barrier, and mucosal and systemic immune functions in a
murine model of non-severe protein-energy-malnutrition. Its potential protection against
Salmonella enterica serovar Typhimurium (S. Typhimurium) infection was also analyzed.serovar Typhimurium (S. Typhimurium) infection was also analyzed.
Methods: Mice were undernourished and divided into 3 groups according to the dietary
supplement received during re-nutrition (milk, probiotic fermented milk or its bacterial free
supernatant) and compared to well-nourished and malnourished mice. They were sacrificed
previous to the re-nutrition and 5 days post re-nutrition. The phagocytic activity of
macrophages from spleen and peritoneum and the changes in the intestinal histology and
microbiota were evaluated. Different immune cell populations and cytokine productions
were analyzed in the small intestine tissues. The effect of the re-nutrition supplements on
the systemic immunity using OVA antigen and against an infection with S. Typhimurium
was also studied.
was also studied.
supplement received during re-nutrition (milk, probiotic fermented milk or its bacterial free
supernatant) and compared to well-nourished and malnourished mice. They were sacrificed
previous to the re-nutrition and 5 days post re-nutrition. The phagocytic activity of
macrophages from spleen and peritoneum and the changes in the intestinal histology and
microbiota were evaluated. Different immune cell populations and cytokine productions
were analyzed in the small intestine tissues. The effect of the re-nutrition supplements on
the systemic immunity using OVA antigen and against an infection with S. Typhimurium
was also studied.
was also studied.
Mice were undernourished and divided into 3 groups according to the dietary
supplement received during re-nutrition (milk, probiotic fermented milk or its bacterial free
supernatant) and compared to well-nourished and malnourished mice. They were sacrificed
previous to the re-nutrition and 5 days post re-nutrition. The phagocytic activity of
macrophages from spleen and peritoneum and the changes in the intestinal histology and
microbiota were evaluated. Different immune cell populations and cytokine productions
were analyzed in the small intestine tissues. The effect of the re-nutrition supplements on
the systemic immunity using OVA antigen and against an infection with S. Typhimurium
was also studied.
was also studied.
S. Typhimurium
was also studied.
Results: Probiotic fermented milk was the most effective re-nutrition diet that improved the
intestinal microbiota. Its administration also increased the number of IgA+ cells,
macrophages and dendritic cells. The production of different cytokine (IFN-ã, TNF-á, IL-
12) by these cells and the phagocytic activity in peritoneum and spleen was also increased.
This re-nutrition diet also stimulated the systemic immune response against OVA antigen
which was diminished after the malnutrition period and also improved the host response
against S. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and the
12) by these cells and the phagocytic activity in peritoneum and spleen was also increased.
This re-nutrition diet also stimulated the systemic immune response against OVA antigen
which was diminished after the malnutrition period and also improved the host response
against S. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and the
intestinal microbiota. Its administration also increased the number of IgA+ cells,
macrophages and dendritic cells. The production of different cytokine (IFN-ã, TNF-á, IL-
12) by these cells and the phagocytic activity in peritoneum and spleen was also increased.
This re-nutrition diet also stimulated the systemic immune response against OVA antigen
which was diminished after the malnutrition period and also improved the host response
against S. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and the
12) by these cells and the phagocytic activity in peritoneum and spleen was also increased.
This re-nutrition diet also stimulated the systemic immune response against OVA antigen
which was diminished after the malnutrition period and also improved the host response
against S. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and the
Probiotic fermented milk was the most effective re-nutrition diet that improved the
intestinal microbiota. Its administration also increased the number of IgA+ cells,
macrophages and dendritic cells. The production of different cytokine (IFN-ã, TNF-á, IL-
12) by these cells and the phagocytic activity in peritoneum and spleen was also increased.
This re-nutrition diet also stimulated the systemic immune response against OVA antigen
which was diminished after the malnutrition period and also improved the host response
against S. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and the
12) by these cells and the phagocytic activity in peritoneum and spleen was also increased.
This re-nutrition diet also stimulated the systemic immune response against OVA antigen
which was diminished after the malnutrition period and also improved the host response
against S. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and the
ã, TNF-á, IL-
12) by these cells and the phagocytic activity in peritoneum and spleen was also increased.
This re-nutrition diet also stimulated the systemic immune response against OVA antigen
which was diminished after the malnutrition period and also improved the host response
against S. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and theS. Typhimurium, decreasing the spread of pathogenic bacteria to the liver and the
spleen. The importance of the metabolites released during milk fermentation was also
demonstrated through the analysis of the bacterial free supernatant obtained from the
probiotic fermented milk, but the whole product showed the best effects in the parameters
evaluated in this study.
Conclusions: The administration of probiotic fermented milk as a dietary supplement
during the re-nutrition process in a murine immunodeficiency model by malnutrition could
be a good adjuvant diet to improve the gut and systemic immune response for the
protection against Salmonella infection.
during the re-nutrition process in a murine immunodeficiency model by malnutrition could
be a good adjuvant diet to improve the gut and systemic immune response for the
protection against Salmonella infection.
: The administration of probiotic fermented milk as a dietary supplement
during the re-nutrition process in a murine immunodeficiency model by malnutrition could
be a good adjuvant diet to improve the gut and systemic immune response for the
protection against Salmonella infection.Salmonella infection.