Pharmacological regulation of mitochondrial nitric oxide synthase

VALDEZ LB; LORES ARNAIZ S; BUSTAMANTE J; ALVAREZ S; BOVERIS AD; NAVARRO A; BOVERIS A

Proceedings of the XI Biennial Meeting of the Society for Free Radical Research International

Monduzzi Editore SpA - Medimond Inc

Lugar: Bologna, Italy; Año: 2002; p. 649 - 652

The activity of mtNOS can be modulated pharmacologically. Chlorpromazine (one dose 10 mg/kg, 1h) inhibited 36-48% mitochondrial NO production, increased 25-38% O2 uptake and did not modify the amount of mtNOS protein detected by Western blot. Enalapril (30 mg/kg/day, up to 15 d) increased NO production in kidney, liver and heart mitochondria. In kidney mitochondria, mtNOS activity was 5 times increased and mtNOS protein 125% increased.