INVESTIGADORES
VALDEZ Laura Beatriz
artículos
Título:
Nitric oxide and superoxide radical production by human mononuclear leukocytes.
Autor/es:
VALDEZ LB; BOVERIS A
Revista:
Antioxid Redox Signal
Editorial:
Mary Ann Liebert, Inc.
Referencias:
Año: 2001 vol. 3 p. 505 - 513
Resumen:
Human mononuclear cells (90% lymphocytes, 9% monocytes, and 1%
polymorphonuclear leukocytes) produced spontaneously in resting state
0.11+/-0.01 nmol of nitric oxide (NO)/min/10(6) cells and 0.25+/-0.02
nmol of superoxide anion (O2-)/min/10(6) cells, as primary products.
When these cells were stimulated with phorbol 12-myristate 13-acetate
(PMA), the NO and O2- production increased by 82% and 204% to
0.25+/-0.02 nmol of NO/min/10(6) cells and 0.76+/-0.12 nmol of
O2-/min/10(6) cells, respectively. Oxygen uptake reasonably accounted
for the sum of the rates of NO and hydrogen peroxide (H2O2), the latter
calculated as 0.5 O2- production, in nonstimulated and in
PMA-stimulated cells. H2O2 and peroxynitrite formation were detected
and measured as secondary products of the primary products O2- and NO.
An original assay to determine H2O2 steady-state concentration and
production rates is described. The determined production rates of the
involved reactive species are in good agreement with known chemical
equations. It is apparent that NO and O2- production by human
mononuclear cells may constitute the basis of intercellular signaling
and cell toxicity.