INVESTIGADORES
VALDEZ Laura Beatriz
artículos
Título:
Inhibition by wine polyphenols of peroxynitrite initiated chemiluminescence and NADH oxidation
Autor/es:
BOVERIS A; VALDEZ LB; ALVAREZ S
Revista:
Ann N Y Acad Sci
Editorial:
The New York Academy of Science
Referencias:
Año: 2002 vol. 957 p. 90 - 102
Resumen:
Peroxynitrite (ONOO(-)) is a powerful oxidant produced by neutrophils,
macrophages, and lymphocytes as a signaling and cytotoxic molecule from
their primary production of nitric oxide (NO) and superoxide anion
(O(2)(-)). In the vascular space, ONOO(-) will likely oxidize
lipoproteins and promote atherogenesis. Pure wine flavonoids (catechin,
epicatechin, myricetin), hydroxycinnamates (caffeic acid, ferulic acid,
chlorogenic acid), and plain Argentine red wines were assayed as
ONOO(-) scavengers in two assays: (a) ONOO(-)-initiated
chemiluminescence and (b) ONOO(-)-dependent oxidation. The assayed
polyphenols as well as the red wines were effective inhibitors of the
ONOO(-)-driven oxidation reactions. Fifty percent of the pure
substances were observed in the range of 30-300 microM and in the case
of red wines with the equivalent of 80-120 microM of flavonoids. The
amphipatic nature of wine polyphenols will lead to their accumulation
at the lipoprotein surface, according to the Gibbs adsorption equation,
where they are likely to prevent ONOO(-)-induced tyrosine nitration and
LDL modification.