INVESTIGADORES
VALDEZ Laura Beatriz
artículos
Título:
Kidney mitochondrial nitric oxide synthase
Autor/es:
BOVERIS A; VALDEZ LB; ALVAREZ S; ZAOBORNYJ T; BOVERIS AD; NAVARRO A
Revista:
Antioxid Redox Signal
Editorial:
Ann Liebert, Inc.
Referencias:
Año: 2003 vol. 5 p. 265 - 271
Resumen:
Nitric oxide synthase activity was recognized in rat renal cortex
mitochondria (mtNOS) with nitric oxide (NO) production rates of
0.14-0.78 nmol/min/mg of protein. Rat pretreatment with enalapril (30
mg/kg/day i.p., up to 15 days) increased NO production in kidney,
liver, and heart mitochondria. In kidney, mtNOS activity and mtNOS
protein, measured by western blot densitometry, were 5 and 2.3 times
increased, respectively. Electron paramagnetic resonance analysis with
the probe N-methyl-D-glucamine dithiocarbamate/FeSO(4) detected NO
production in mitochondria isolated from enalapril-treated rats, but
not in control untreated animals. Polyclonal antibodies anti-iNOS and
anti-nNOS detected kidney mtNOS in western blots and inhibited mtNOS
biochemical activity. The enzymatic activity of kidney mtNOS generates
intramitochondrial NO concentrations that regulate mitochondrial
functions: state 3 respiration was decreased by 12-28%, and state 4
hydrogen peroxide production was increased 12-35%.