Role of galectin-1 on intestinal epithelial cells in inflammatory settings

MUGLIA CECILIA; PAPA GOBBI RODRIGO; SMALDINI PAOLA; ORSINI DELGADO MARIA LUCIA; SAMBUELLI ALICIA; ROCCA ANDRES; TOSCANO MARTA; RABINOVICH GABRIEL; DOCENA GUILLERMO H.

Buenos Aires

Congreso; First International Congress of LASID FAIC SAI, IV Meeting LASID and LXIII Congreso Anual de la Sociedad Argentina de Inmunología; 2015

SAI

Galectins play key roles in cell survival and in inflammation.Here we analyzed the effect of galectin-1 (Gal-1) onthe function ofintestinal epithelial cells (IECs)isolated from healthy and inflamed mucosa.IECs isolated from miceor patients with inflammatory bowel disease (IBD)were incubated with different pro-inflammatory cytokines and Gal-1 binding(flow cytometry), secretion of homeostatic factors (ELISA)and cell viability (AnnexinV/PI, caspase activity and Bcl family members) wereassessed. Experimental models of food allergy and colitis were used to evaluate the in vivo influence of inflammation on Gal-1 properties.We found enhanced binding of Gal-1 to: a) murine IECs exposed to IL-1β (P