Pro-inflammatory effect of volcanic ash in a colitis model

ORSINI DELGADO MARIA LUCIA; SAMBUELLI ALICIA; PAPPA GOBI RODRIGO; GIL ANÍBAL; NEGREIRA SILVIA; GONCALVES SILVINA; HUERNOS SERGIO; BELLICOSO MARICEL; TIRADO PABLO; CHAVERO PAULA; DOCENA GUILLERMO H.

Barcelona

Congreso; Inflammatory Bowel Diseases X Congress of ECCO; 2015

ECCO

BackgroundIt is widely accepted a role for environmental factors in the IBD. Urban pollutants were associated with hospitalization rates and early IBD debut. Intestinal exposure to particulate matter (PM) has a proinflammatory effect inducing permeability and TNFα. Volcanic pollution, implicated in varied health impairments, was not studied in IBD. Increase PM remained after Iceland eruptions by ash resuspension (Thorsteinsson T,2012). High IBD incidences were reported in Iceland (volcanic), Faroe Islands and N. Zealand. During devastating Puyehue (Patagonian volcano, 2011) eruptions, increased PM by ash-carrying winds were detected in Buenos Aires (Argentina), as well as refractoriness in hospitalized IBD. Hypothesis: volcanic ash (VA) have a pro-inflammatory role in IBD. Aim: to study the effect of VAin a colitis mouse model.MethodsBALB/c mice received drinking water with VA for 14 days. On day 7 different inflammatory stimuli were intrarectally administered: TNBS, Flagellin (FliC) or ethanol (EtOH), as vehicle. On day 14 mice were sacrificed and the colon studied (weight, length, histology, ZO-1 immunofluorescence, gene expression of IL1 β, IL6, TNFα, Ccl20, IFN-γ, T-bet, IL17A, RORγt by qPCRResultsWe found a marked weight loss in mice receiving drinking water+VA vs mice with water or EtOH. Mice receiving VA showed a greater ratio weight/length of colon compared to colon (EtOH/EtOH+VA 28.4±4.4 vs 31.3±2.4.0, TNBS/TNBS+VA 28.3±0.1 vs 33.7±4.2, FliC/FliC+VA 24.7±4.01 vs 31.5±5.9). Histological activity index: EtOH/EtOH+VA: 1vs3 (p