In silico prediction of cross-reactive epitopes of the major soybean allergen Gly m Bd 30K (P34) with bovine caseins and their analysis by immunochemical methods

CANDREVA A; PARISI GUSTAVO; DOCENA GUILLERMO H.; PETRUCCELLI SILVANA

Oro Verde

Congreso; Tercer Congreso Argentina de Bioinformática y Biología Computacional; 2012

Cow’s milk allergy (CMA) constitutes the main food allergy in Argentina. The nutritional substitutes mostly used are soy-based formulas; however, 15-40% of the patients do not tolerate soybean milk. The molecular bases of these reactions are not fully understood. Our group has demonstrated that patients allergic to cow´s milk proteins (CMP) have IgE sera that recognized 7S and 11S storage proteins and also the major soy allergen P34. Besides, a panel of casein specific monoclonal antibodies also recognized these soybean proteins, suggesting co-recognized epitopes among bovine caseins and these seed proteins. Furthermore, we have shown that CMP-sensitized mice developed hypersensitivity symptoms following exposure to total soybean proteins and to P34 recombinant protein, thus demonstrating the clinical impact of the immunochemical cross-reactivity. Although these results indicate the presence of co-recognized epitopes servers predicting allergenic proteins and for mapping IgE epitopes (SDAP, AlgPred, immuneepitope database) fail to predict common epitopes among bovine caseins and P34