INVESTIGADORES
DOCENA Guillermo Horacio
congresos y reuniones científicas
Título:
Identification of soybean proteins that show cross-allergenicity with cow milk caseins
Autor/es:
CURCIARELLO RENATA; PETRUCCELLI SILVANA; FOSSATI C; DOCENA GUILLERMO H.
Reunión:
Congreso; 13th International Congress of Immunology; 2007
Resumen:
Cow milk allergy (CMA) constitutes the main food allergy observed in the pediatric population in Argentina.
Besides, soy-based formulas are the nutritional substitute mostly
employed as treatment. However, adverse reactions have been reported in
40% of the treated patients which might be caused by cross-reactive
components that share immunodominat epitopes. Our group has previously
described the cross-reactivity between the glycinin A5A4B3 and bovine
caseins. We aimed to identify additional cross-allergenic soy components
since residual immunochemical cross-reactivity was detected when Glycine max L.merr Raiden, a soy variety that naturally lacks A5A4B3, was studied. Employing immunoenzimatic
methods (immunoblotting and ELISA), cow milk specific antibodies
(rabbit sera, monoclonal antibodies, IgEcontaining sera from cow milk
allergic) and several antigens (soybean purified proteins, recombinant
full-length and fragmented proteins) we identified the a and b subunits of b-conglycinin
(a soybean 7S globulin) as new potential soybean allergens that
cross-react with cows milk proteins. This immunochemical detected
cross-reactivity may be responsible for the intolerance observed during
the treatment of cow milk allergic children. Further studies will be
necessary to identify inmunodominant epitopes in soy globulins, in
order to design a strategy to reduce the allergenicity of the soybean
formula.