INVESTIGADORES
DOCENA Guillermo Horacio
artículos
Título:
In vivo evidence of cross-reactivity between cow’s milk and soybean proteins in a mouse model of food allergy
Autor/es:
SMALDINI PAOLA; CURCIARELLO RENATA; CANDREVA ANGELA; REY MARIA AMANDA; FOSSATI ALBERTO; PETRUCCELLI SILVANA; DOCENA GUILLERMO H.
Revista:
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
Editorial:
KARGER
Referencias:
Lugar: BASEL; Año: 2011 vol. 158 p. 335 - 346
ISSN:
1018-2438
Resumen:
BACKGROUND: Cow´s milk allergy (CMA) is an important problem worldwide and the development of an in vivo system to study substitute formulae and immunotherapeutic strategies is of interest. Intolerance to soybean formula has been described in CMA patients but it is not fully understood. In this work we used a food allergy model in BALC/c mice to study the cross-reactivity between cow´s milk (CMP) and soy proteins (SP). METHODS: Mice were orally sensitised and then challenged with CMP or SP. Symptoms elicited, plasma histamine, humoral and cellular immune response were analysed. Th1- and Th2-associated cytokines and transcription factors were assessed at mucosal sites and in splenocytes. Cutaneous tests were also performed. RESULTS: We found that immediate symptoms elicited in CMP-sensitised mice orally challenged with SP were consistent with an increase in plasma histamine concentration. Serum level of CMP-specific IgE and IgG1 antibodies were increased. CMP-specific IgE and IgG1 antibodies also recognised soy proteins. Splenocytes and mesenteric lymph node cells incubated with CMP or SP secreted IL-5 and IL-13. mRNA expression of Th2-associated genes (IL-5, IL-13, and GATA-3) was up-regulated in mucosal samples. Besides, sensitised animals exhibited positive cutaneous tests after injection of CMP or SP. CONCLUSIONS: We demonstrate that CMP-sensitised mice, without previous exposure to soy proteins, elicited hypersensitivity signs immediately after the oral administration of SP, suggesting that the immunochemical cross-reactivity might be clinically relevant. This model may provide an approach to further characterise cross-allergenicity phenomena and develop new immunotherapeutic treatments for allergic patients.