Curcumin suppresses p38 MAPK activation, reduces IL-1â and MMP-3, and enhances IL-10 in the mucosa of children and adults with inflammatory bowel disease

JENNY EPSTEIN; GUILLERMO DOCENA; THOMAS T. MACDONALD; IAN SANDERSON

BRITISH JOURNAL OF NUTRITION

CAMBRIDGE UNIV PRESS

Año: 2009 vol. 103 p. 824 - 832

0007-1145

Inflammatory bowel disease (IBD) is a major source of morbidity in children and adults. Its incidence is rising, particularly in young people. IBD carries a lifelong risk of cancer which is proportional to disease duration. Drug and surgical treatments rarely offer cure and often carry a high side effect burden. Dietary therapy is highly effective in selected cases of Crohn’s disease. For these reasons there is much interest in developing novel dietary treatments in IBD. Curcumin, a component of the spice turmeric, and an anti-inflammatory and anti-cancer agent, shows preclinical and clinical potential in IBD. Its mechanisms of action are unknown. Our aim was to assess the effect of curcumin on key disease mediators p38 MAPK, IL-1â, IL-10 and MMP-3 in the gut of children and adults with IBD. Colonic mucosal biopsies and myofibroblasts (CMF) from children and adults with active IBD were cultured ex vivo with curcumin. p38 MAPK, NF-êB and MMP-3 were measured by immunoblotting. IL-1â and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA). We show reduced p38 MAPK activation in curcumin-treated mucosal biopsies, enhanced IL-10 and reduced IL-1â. We demonstrate dose-dependent suppression of MMP-3 in CMF with curcumin. We conclude that curcumin, a naturally occurring food substance with no known human toxicity, holds promise as a novel therapy in IBD.