INVESTIGADORES
DOCENA Guillermo Horacio
artículos
Título:
Serological investigation of latex allergy in Argentina
Autor/es:
DOCENA GUILLERMO H.; FERNANDEZ RITA; OCAMPO MARCELO; FOSSATI ALBERTO
Revista:
ALLERGY AND ASTHMA PROCEEDINGS
Editorial:
OCEAN SIDE PUBLICATIONS INC
Referencias:
Año: 1999 vol. 20 p. 99 - 106
ISSN:
1088-5412
Resumen:
Atopy to latex has been reported in different high-risk groups of subjects in whom it is mainly caused by proteins from natural latex
that are responsible for eliciting a variety of clinical symptoms, some
of them systemic. Thus, identification of subjects sensitized to latex proteins is of great health importance, because it would become possible to advise them to avoid contact with latex-containing products. Protein extracts from ammoniated latex
were prepared by incubation with PBS buffer either with or without
detergents, followed by ultracentrifugation. Three immunoenzymic methods
were developed (EAST, ELISA, and immunoblotting) to detect the presence
of specific IgE antibodies against latex proteins in sera of patients from different groups at risk. The protein content of the latex extracts ranged from 5.3 to 8.8 mg/mL. The prevalence of specific IgE against latex
proteins was 9/28 (32.1%) in children with multiple surgeries, 17/98
(17.3%) in health care workers, and 23/123 (18.6%) in outpatients
assisted at the Allergy
Department. None of the sera belonging to the healthy control group
showed the presence of specific IgE. Therefore, this protein extract
from latex could be used to detect specific IgE antibodies in serum by immunoenzymic methods. Serologic results for latex-specific IgE found are in accordance with those reported in the literature of other countries.