Poly(allylamine)-tripolyphosphate ionic assemblies as nanocarriers: friend or foe?

APUZZO, EUGENIA; AGAZZI MAXIMILIANO; HERRERA SANTIAGO; PICCO, AGUSTIN; RIZZO, GASTÓN PASCUAL; CHAVERO, CAMILA; BIANCHI, DAIANA; SMALDINI, PAOLA; CORTEZ, MARÍA LORENA; MARMISOLLÉ, WALDEMAR; PADULA, GISEL; SEOANE, ANALÍA; ALOMAR, MARIA LIS; FRANCO CABRERIZOAULA; DOCENA GUILLERMO; AZZARONI OMAR

American Chemical Society Applied Bio Materials

ACS Publications

Año: 2022

2576-6422

The design of effective drug nanocarriers that are easy to synthesize, robust, non-toxic, and capable of encapsulating therapeutic agents is a significant challenge in nanomedicine. Polyamine-multivalent molecule nanocomplexes have been proposed as drug carriers due to their simple and all-aqueous manufacturing process. In this study, polyallylamine was ionically assembled with tripolyphosphate anions to form nanocomplexes with a size around 200 nm and a zeta potential of -30 mV. Nanocomplexes exhibit tremendous long-term stability (9 months of storage) and in complex media. They also have a good capacity to encapsulate various proteins as model bioactive cargoes. In addition, biological studies demonstrate that nanocomplexes are not significantly toxic and can be internalized by macrophage cells. Therefore, these nanocomplexes have attractive and promise properties as delivery nanoplatforms for potential therapies based on immune system activation.