Emergence of Pseudomonas aeruginosa Strains Producing Metallo-beta-lactamases of the IMP-15 and VIM-2 types in Mexico

F.QUIÑONES-FALCONI; M. GALICIA-VELASCO; P.MARCHIARO ; V. BALLERINI; M. MUSSI; A. J. VILA; A.M. VIALE; K. BERMEJO-MORALES; A.S.LIMANSKY

CLINICAL MICROBIOLOGY AND INFECTION

WILEY-BLACKWELL PUBLISHING, INC

Lugar: New York; Año: 2010 vol. 16 p. 126 - 131

1198-743X

Eighty-six carbapenem non-susceptible Pseudomonas aeruginosa isolates collected in the National Institute of Respiratory Diseases of Mexico City were screened for the presence of metallo-beta-lactamase (MΒL) activity using both E-test strips and a microbiological assay with EDTA-imipenem. Genomic comparisons and sequence analyses conducted with these isolates revealed the presence of blaVIM-2 in two clonally related isolates, and blaIMP-15 in a clonally unrelated isolate. Both genes were found to be carried by class 1 integrons, and blaIMP-15 was additionally present on a broad host-range plasmid. This is the first report of co-existing P. aeruginosa strains producing different MBLs in a Mexican hospital, highlighting the necessity of appropriate surveillance to prevent dissemination of carbapenem resistance