Biochemical characterization of metallo-beta-lactamase VIM-11 from a Pseudomonas aeruginosa clinical strain

MARCHIARO, P; TOMATIS, P E; MUSSI, M A; PASTERAN, F; VIALE, A M; LIMANSKY, A S; VILA, A J

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

American Society for Microbiology

Lugar: Washington; Año: 2008 vol. 52 p. 2250 - 2252

0066-4804

A detailed biochemical characterization of the Pseudomonas aeruginosa VIM-11 metallo-beta-lactamase (MBL) is reported. The only substitution differentiating VIM-11 from VIM-2 (N165S) promoted a slightly improved catalytic efficiency of the former on 3 out of 12 substrates, notably the bulky cephalosporins. Thus, MBLmediated resistance also may be modulated by remote mutations.