The Met axial ligand determines the redox potential in CuA Sites

LEDESMA, GN; MURGIDA, D; KHOA LY, H; WACKERBARTH, H; ULSTRUP, J; COSTA-FILHO, A; VILA, AJ

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

American Chemical Society.

Lugar: United States; Año: 2007 vol. 129 p. 11884 - 11885

0002-7863

The replacement of the axial methionine ligand in a native CuA protein rendered a series of stable mutants with spectroscopic features of a mixed valence center. The mutations resulted in minor perturbations of the electronic structure of this site but led to significant changes in the redox potential. This suggests that the axial ligand plays a role in tuning the redox potential of CuA, resembling the behavior observed in blue copper sites.