A 1H NMR NOE study on Co(II) stellacyanin: some clues about the structure of the metal site.

ALEJANDRO JOSE VILA

FEBS LETTERS

ELSEVIER SCIENCE BV

Año: 1994 vol. 355 p. 15 - 18

0014-5793

The ?H NMR spectrum of Co(H) stellacyanin is reported, in which four signals not previously observed have been detected. NOE experiments were performed to assign the hyperfine shifted signals corresponding to a Cys and two His residues. Both His residues are solventaccessible and are shown to bind the metal ion through their NJ1 atoms. The j?-CH, Cys proton shifts indicate the presence of a strong axial ligand.