High scleroglucan production by Sclerotium rolfsii: Influence of medium composition.

JI FARINA,; FAUSTINO SINERIZ,; MOLINA, OSCAR; PEROTTI, NI

BIOTECHNOLOGY LETTERS

SPRINGER

Lugar: Dordrecht; Año: 1998 vol. 20 p. 825 - 831

0141-5492

ABSTRACT: Scleroglucan production by Sclerotium rolfsii was markedly affected by the C-source concentration, showing a highest value with 150 g sucrose l?1. Production was also influenced by the N-source, being considerably higher in media containing NO3? than in those containing NH4, which had a clear inhibitory effect. Once defined the optimum culture medium composition, the highest exopolysaccharide production (ca. 26 g scleroglucan l?1) was achieved after 72 h of fermentation at shake flask scale. High values of yield (Yp/c = 0.49), productivity (Pr = 0.365 g l?1 h?1) and specific productivity (Pr/x = 0.031 g (g biomass)?1 h?1) were observed, and productivity was 1.5 times further increased by scalling-up to fermenter scale. Addition of L-threonine, sunflower oil and ascorbic acid diminished exopolysaccharide production. Rapid Science Ltd. 1998