Production of fructooligosaccharides by Aureobasidium sp. ATCC 20524 in stirred tank reactor

SALINAS MARTÍN ANDRÉS; PUCCI, CLAUDIO A.; RB LUQUE,; NORA I PEROTTI

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Tucumán; Año: 2006 vol. 30 p. 169 - 169

0327-9545

PRODUCTION OF FRUCTOOLIGOSACCHARIDES BY Aureobasidium sp. ATCC 20524 IN STIRRED TANK REACTOR Salinas M2, , Pucci C1, Luque R1,2, Perotti N1,2. 1 Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. 2 PROIMI-CONICET. Av. Belgrano y Pje. Caseros, San Miguel de Tucumán (CP 4000), Tucumán. E-mail: martin.salinas@proimi.org.ar Introduction. Fructooligosaccharides (FOS) are considered both alimentary additives and nutraceutics. Several studies have demonstrated that prebiotic properties of these sugars favor the normal functioning of the digestive system. Objective. The objective of this work was to produce FOS by Aureobasidium sp. ATCC 20524 in submerged culture on bioreactor using saccharose as carbon source. Kinetics parameters of growth, fructosyltransferasa enzymatic activity, and synthesis of FOS were studied. Materials and Methods. The bioproduction was carried out in a stirred tank reactor, on a volume of 1 liter. The sugars were determinated by HPLC and the enzymatic activity was carried out by the method of Glucose-oxidase. Results and Conclusions. Maximum values of enzymatic activity (202.27 U/mL) and specific enzymatic activity (1.88 U/mL) were obtained on the fifth day. Maximum values of produced FOS were: 51.8 g/L for 1-kestose and 74.6 g/L for nystose. The values of enzymatic activity were higher than the ones found in the bibliography