Cationic polyelectrolytes–lipases complexes formation as tool for recovery of

G. BASSANI; B. FARRUGGIA; G. PICO

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2011 vol. 49 p. 351 - 355

0141-8130

In the present paper the formation of complexes between positively charge polyelectrolyte    (polyethyleneimine and chitosan) and Candida rugosa lipase from a crude extract and porcine lipase  from pancreas commercial homogenate preparations were analyzed. The solubility of lipases–cationicpolyelectrolytes formation was dependent on: polyelectrolyte densities electrical charge, polyelectrolyte  and enzyme concentrations and salts present in the solution. The lipase was recovered from the nonsoluble  complex by adding of NaCl at a given pH. Although the polyelectrolytes did not affect lipase  biological activity, both of them produced good enzyme recovery (>90%); however, purification factors  were low. This methodology appears to be a good previous prepurification and concentration method, using, low-cost polymers, allows the design of a purification method where the protein of interest is  present in a large volume with respect to the small amount of polyelectrolyte added.Candida rugosa lipase from a crude extract and porcine lipase  from pancreas commercial homogenate preparations were analyzed. The solubility of lipases–cationicpolyelectrolytes formation was dependent on: polyelectrolyte densities electrical charge, polyelectrolyte  and enzyme concentrations and salts present in the solution. The lipase was recovered from the nonsoluble  complex by adding of NaCl at a given pH. Although the polyelectrolytes did not affect lipase  biological activity, both of them produced good enzyme recovery (>90%); however, purification factors  were low. This methodology appears to be a good previous prepurification and concentration method, using, low-cost polymers, allows the design of a purification method where the protein of interest is  present in a large volume with respect to the small amount of polyelectrolyte added.