INMIBO ( EX - PROPLAME) 14614
INSTITUTO DE MICOLOGIA Y BOTANICA
Unidad Ejecutora - UE
artículos
Título:
AFLP characterization in pathogenic and coprophilous fungi
Autor/es:
ISABEL E. CINTO; ALEXANDRA GOTTLIEB; MARCELA GALLY; MARIA E RANALLI; ARACELI M. RAMOS
Revista:
MYCOTAXON
Editorial:
MYCOTAXON LTD
Referencias:
Lugar: NY; Año: 2009 vol. 110 p. 81 - 87
ISSN:
0093-4666
Resumen:
Abstract The objective of this study was to ascertain the usefulness of the AFLPtechnique in assessing genetic diversity among 47 strains belonging to three Ascomycotagenera and as a tool for solving taxonomic problems in related morphological species.Four MseI +1 primers were assayed in combination with two EcoRI +2 and four EcoRI+3 primers. In the present study both +2 and +3 EcoRI primers were informative,but EcoRI +2 produced profiles with high complexity. The addition of the extraselective nucleotide reduced the complexity of the banding patterns generating easilyreadable patterns to evaluate genetic diversity within and among species. Of the threeascomycetous genera assessed in this study, Colletotrichum (Glomerellaceae) presentedthe highest proportion of polymorphic AFLP loci, followed in order by Iodophanus(Pezizaceae) and Saccobolus (Ascobolaceae).
