THYROID HORMONE TRANSPORT AND METABOLISM IN MICE DENDRITIC CELLS: EXPRESSION OF GENES INVOLVED.

GIGENA N; ALAMINO VA; MONTESINOS MM; NAZAR M; MASINI-REPISO AM; CREMASCHI, G; PELLIZAS CG

Florianópolis

Congreso; XV Congress of the Latin American Thyroid Society (LATS); 2013

Latin American Thyroid Society (LATS)

Background: We reported thyroid hormone (TH) action at dendritic cell (DC) level (Mascanfroni et al. FASEB J 2008, 22:1032 and J Biol Chem 2010, 285:9569; Montesinos et al. Steroids 2012, 77:67). However, the mechanisms involved in TH transport and metabolism in DC are yet unknown. Objetives: To explore in DC the expression of: 1) TH transporters: Monocarboxylate Transporter 8 (MCT-8), MCT-10 and Organic Anion Transporting Polypeptide 1C1 (OATP1C1), 2) Deiodinases of iodothyronines: Dio 1, 2 and 3, and T3-induced effects on that expression. Methods: mice immature DC were maturated with T3 (10 nM) or LPS (100 ng/ml) for 18 h. The presence and levels of the mRNAs coding the proteins mentioned above were evaluated through conventional RT-PCR and Real-time RT-PCR. The identity of each mRNA was confirmed by Nested RT-PCR and Genetic Sequencing. Results: 1) DC express only mRNA-MCT-10, not MCT-8 or OATP1C1, and the expression of mRNA-MCT-10 is higher in matured DCs than in immature DCs. 2) DC express mRNA-Dio2 and Dio 3 but not Dio 1, and T3 increased mRNA-Dio3 levels. Conclusions: This work revealed mRNA-MCT-10 expression, suggesting that the coded protein functions as a TH transporter in DC. Besides, the expression of mRNA-Dio 2 and Dio 3 suggests that their proteins may act in the activation-inactivation of TH in DC. In addition, T3 participates in the regulation of Dio3 expression. Although additional studies at protein level are necessary to further disclose these issues, our findings provide the first evidences of TH transport and metabolism in DC