 P75NTR REGULATES MONONUCLEAR PHAGOCYTIC CELLS RECRUITMENT IN A MOUSE MODEL OF CHOROIDAL NEOVASCULARIZATION.

TOVO, A; LUNA JD; ANASTASIA A; VAGLIENTI MV; SARAGOVI, HU; BARCELONA PF; SUBIRADA PV; RIBOTTA, NA; SANCHEZ, MC

Buenos Aires

Congreso; SAN 2022 (XXXV Congreso Anual de Sociedad Argentina de Investigación en Neurociencias).; 2022

Sociedad Argentina de Investigación en Neurociencias

With the advent of old age, pathologies appear that drive on a decline of the neuronal function in the eye. Particularly in the wet age-related macular degeneration, retinal pigmented epithelium (RPE) cells are known to diminish their trophic support and mononuclear phagocytic cells (MPCs) accumulation, which leading to an inflammatory chronic state promote choroidal neovascularization (CNV) leading to photoreceptor degeneration. The mechanisms involved on the MPCs collaborate in the development of vascular growth remain cryptic. The p75 neurotrophin receptor (p75NTR) is one of the main surface proteins involved in the transduction of death signals and also vascular changes. Here, we inquire about the role of p75NTR in the recruitment of MPCs to the injured area in a mouse model of laser-induced CNV. Western blot assay showed increased protein expression of p75NTR in RPE-Choroids 4 days after laser. Also Confocal images of RPE-choroid wholemounts of this CNV mice stained with F4/80 evidenced presence of p75NTR in MPCs. Although similar high area occupied by MPCs was observed in p75NTR KO mice respect to WT mice, the flow cytometry assay revealed a decrease number of MPCs in RPE-Choroids and retinas of p75NTR KO mice respect to WT mice. Pharmacological inhibition of p75NTR in vivo also achieved a reduction in the number of MPCs in RPE-Choroids and retinas, suggesting that p75NTR is involved in MPCs recruitment.