Carbohydrated components of Excretory-Secretory Products from Fasciola hepatica induce eosinophils apoptosis by a caspases-dependent mechanism

. SERRADELL M DEL C, GUASCONI L, CERVI L, CHIAPELLO L AND MASIH DT

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

Elsevier

Lugar: Amsterdam. Holanda; Año: 2006

0165-2427

Eosinophils (Eo) are known to be important effector cells in the host defense against helminth parasite. Excretory-secretory products (ESP) released by helminths have shown  wide immunomodulatory properties, such as the induction of cellular apoptosis. We investigated the ability of ESP from Fasciola hepatica to induce Eo apoptosis. In this work, we observed that ESP induced an early apoptosis of rat peritoneal eosinophils and that  this phenomenon was time- and concentration-dependent. Furthermore, we demonstrated  that activation of protein tyrosine kinases (TyrK) and caspases were necessary to mediate  the Eo apoptosis induced by the ESP, and that carbohydrate components present in these antigens were involved in this effect. We have described for the first time the ability of ESP from F. hepatica to modify the viability of Eo by apoptosis induction. Besides that, we have observed Eo apoptosis in the liver of rats 21 days after F. hepatica infection. The diminution in Eo survival in early infection could be a parasite strategy in order to prevent a host immune response.helminth parasite. Excretory-secretory products (ESP) released by helminths have shown  wide immunomodulatory properties, such as the induction of cellular apoptosis. We investigated the ability of ESP from Fasciola hepatica to induce Eo apoptosis. In this work, we observed that ESP induced an early apoptosis of rat peritoneal eosinophils and that  this phenomenon was time- and concentration-dependent. Furthermore, we demonstrated  that activation of protein tyrosine kinases (TyrK) and caspases were necessary to mediate  the Eo apoptosis induced by the ESP, and that carbohydrate components present in these antigens were involved in this effect. We have described for the first time the ability of ESP from F. hepatica to modify the viability of Eo by apoptosis induction. Besides that, we have observed Eo apoptosis in the liver of rats 21 days after F. hepatica infection. The diminution in Eo survival in early infection could be a parasite strategy in order to prevent a host immune response.