Role of Alkaline/Neutral invertases in Anabaena strains in response to salt stress

C. NISHI; W.A. VARGAS; H.G. PONTIS; G.L. SALERNO

Villa Carlos Paz, Córdoba, Argentina

Congreso; XLIV Reunión Anual de la SAIB (Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2008

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

In Anabaena (Nostoc) sp. PCC 7120 we have identified and biochemically characterized two alkaline/netural invertase genes coding (invA and invB) for proteins of about 53 - 55 kDa. the aim of this work was to investigate if these two enzymes play different roles in  Anabaena filaments. By expression analysis and localization of  InvA and InvB in the nitrogen-fixing filament cells, supported the role of inv activity in nitrogen fixation, particularly, InvB activity. On the other hand a key function of alkaline/netural invertases in salt tolerance was demonstrated by transcriptional, translational and activity level. The impair of alkaline/netural invertase activity affect salt tolerance. We conclude that a high sucrose turnover is important to cope salt stress.