Transformed soybean (Glycine max) roots as a tool for the study of the arbuscular mycorrhizal symbiosis

LAURA FERNÁNDEZ; VANESA SILVANI; JOSEFINA BOMPADRE; MARIANA PÉRGOLA; ALICIA GODEAS

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Springer Netherlands

Año: 2009 vol. 25 p. 1857 - 1863

0959-3993

Ri T-DNA transformed roots have been usedeffectively in studying the interaction between variousplant hosts and arbuscular mycorrhizal (AM) fungi. Weinvestigated the in vitro monoxenic symbiosis between theAM fungus Glomus intraradices and transformed soybeanroots (TSRs). Comparisons were made between TSR systemand plants of the same genotype. The extraradicalfungal structures generated in vitro culture showed normaldevelopment. Straight runner hyphae branched into shortsimple branched absorbing structures and spores wereinitiated. AM symbiosis was confirmed by the presence ofarbuscules and vesicles in cortical cells of the TSRs. Thefrequency of intraradical colonization in TSRs was higherthan in plants grown in soil, whereas the intensity values ofintraradical colonization in TSR cultures were similar tothose in whole plants. These results show that TSR cultureswere able to support the growth and characteristic developmentof G. intraradices.