Evaluation of indigenous bacterial strains for biocontrol of the frogeye leaf spot of soybean caused by Cercospora sojina

SIMONETTI, ESTER; CARMONA, MARCELO; SCANDIANI, MARÍA; GARCÍA, AUGUSTO; LUQUE, ALICIA; CORREA, OLGA; BALESTRASSE, KARINA

LETTERS IN APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 vol. 55 p. 170 - 173

0266-8254

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Methods and Results: From cultures of each bacterial strain, cell suspensions and cell-free supernatants were obtained and assayed to determine their antifungal activity against C. sojina. Both, mycelial growth and spore germination in vitro, were more strongly inhibited by bacterial cell suspensions than by cell-free supernatants. The Bacillus strains BNM122 and BNM340 inhibited the fungal growth to a similar degree (I ~52?53%), while cells from P. fluorescens BNM297 caused a lesser reduction (I ~32?34%) of the fungus colony diameter. The foliar application of the two Bacillus strains on soybean seedlings, under greenhouse conditions, significantly reduced the disease severity with respect to control soybean seedlings and those sprayed with BNM297. This last bacterial strain was not effective in controlling FLS in vivo. Conclusions: Our data demonstrate that the application of antagonistic bacteria may be a promising and environmentally friendly alternative to control the FLS of soybean. Significance and Impact of the Study: To our knowledge, this is the first report of biological control of C. sojina by using native Bacillus strains