Effect of Ilex paraguariensis (Mate) aqueous extracts and chlorogenic acid on the antioxidant enzymes (SOD, CAT and Px) in rat submandibulary glands

ANESINI CLAUDIA; COGOI LAURA; MARTINO RENZO; FILIP ROSANA

Londres

Congreso; 12th World Congress on Scientific Program ALTERNATIVE MEDICINE; 2018

Statement of Problem: Reactive oxygen species (ROS) are well known inducers of cellular and tissue pathogenesis leadingto numerous disorders including periodontal diseases. Antioxidant enzymes (AE) such as superoxide dismutase (SOD),peroxidase (Px) and catalase (CAT), play a protective role in mouth oxidative physiological and pathological processsuch as inflammation, infections and cancer. Ilex paraguariensis (mate tree) is a plant cultivated in South America. Theleaves and stems are industrialized to obtain the "yerba mate" product consumed in the region as a tea-like beverage. Thisspecies is recognized worldwide for its nutritional, functional and medicinal properties and exported to over 40 countries.The stimulant, diuretic, antioxidant, hypocholesterolemic, slimming effects, are attributed to the bioactive compounds(methylxanthines, polyphenols and saponins). Polyphenols (caffeoyl derivative compounds) like chlorogenic acid (CL)have demonstrated antioxidant activity. Previous reports indicated that aqueous extracts of I. paraguariensis (AE) exerted apositive effect on the human oral health. The objective of this work was to determine in vitro, the effect of AE and CL uponSOD, CAT and Px secretion, using a rat submandibulary glands model.Methodology: AE were prepared and analyzed by HPLC. Submandibular glands were removed from the female albinoWistar rats. Different concentrations of AE and CL were assayed and SOD, CAT and Px secretion were determined.Findings: AE and CL produced a secretion increase (SI) on SOD, CAT and Px, with different patterns. The maximum valuefor SI was AE: 700 % (SOD), 60% (CAT) and 66% (Px). CL: 100% (SOD). A biphasic response for CAT and Px was observed.Conclusion & Significance: AE and CL are promising agents for the prevention or treatment of oral pathologies in whichoxidative stress plays an important role and the concentrations to be used could be estimated according to the resultsobtained in this work.