MAMUSHKA-LIKE STRUCTURED BIOMATERIALS WITH ANTIMICROBIAL ACTIVITY

BELLINO MARTIN G.; ANTEZANA PABLO E.; MUNICOY SOFIA; DESIMONE MARTIN F

CABA

Jornada; XX Jornadas Anuales de la Sociedad Argentina de Biología (SAB)-XVII Jornadas de la Sociedad Uruguaya de Biociencias (SUB), Segundas Jornadas Rioplatenses de Biología; 2018

Sociedad Argentina de Biología

Hydrogels have been demonstrated to be one of the most promising materials in wound care, as they effectively prevent contamination and a greater progression of infection to deeper tissues. Incorporation of lipid vesicles (liposomes) in these polymeric hydrogel matrices have appeared as an attractive approach for minimizing burst release effects and improving localization of the drugs supplied, protecting them at the same time from their degradation. On the other hand, silver nanoparticles (AgNPs) are very interesting as antimicrobial agents and are widely used in different biomedical applications, such as in the treatment of wound and burn infections. In this work, antimicrobial mamushka-like hydrogels have been developed by the encapsulation of AgNPs into liposomes and the later incorporation of these assemblies in collagen gels (Col-L-AgNPs), to study their bactericidal effects on the growth of Gram positive (Staphylococcus aureus) and negative (Pseudomonas aeruginosa) bacteria. In this sense, both type of microorganism were incubated at 37°C in presence of the different gels and after 24 h were sown on Petri dishes to study the viability of bacteria at 24 h. As a result, the growth of Gram positive and negative bacteria was strongly inhibited in presence of Col-L-Ag, possibly due to a controlled release of Ag+ ions over time. Therefore, these liposomal hydrogels loaded with AgNPs are potentially effective as broad-spectrum antimicrobial platforms