. Rosuvastatin promotes a cardioprotective metabolic environment in hearts subject to ischemia-reperfusion

MESTRE CORDERO, VICTORIA EVANGELINA; D VELEZ; M.G MARINA PRENDES; HERMANN R; JULIANA PEREGO

Congreso; IMBSCongress 2018; 2018

Previously in our laboratory we investigated the cardioprotective actions of rosuvastatin (R) in hearts subjected to ischemia-reperfusion (I-RP). We could see that they were mediated, at least in part, by the activation of the Akt protein which promots the inhibition of GSK3B by inducing the attenuation of the Mitochondrial Permeability Transition Pore (MPTP) opening. In the present work we set out to investigate the metabolic environment involved in the aforementioned events and mitochondrial morphology in Langendorff perfused rat hearts subject to I-RP. Wistar female rats (250-300g body weight) fed ad libitum were used. R (3uM) and wortmannin (W 100nM), Akt inhibitor, were added 10 and 15 min before global I respectively. Hearts were subjected to 25 min of I and 60 min of RP. Tissue samples were taken at the end of stabilization and ischemia to determinate lactic acid and glycogen content, electronic microscopies were performed at the same time for assess mitochondrial damage. ANOVA (n=6/group). Lactic acid content were meaningfully lower in those hearts treated with R (μmoles/g dw): C (control): 146,28±6,60**; R: 56,68±6.77; W: 65,31±5,22; R+W: 61,35±4,10 (**p