Dietary zinc restriction during early stages of life programs alterations in renal renin-angiotensin system in adult rats

GOBETTO MN; ALADRO M; GIRONACCI M; ARRANZ C; MENDES GARRIDO F; SARAVIA M; CANIFFI C ; TOMAT A; BANDIERA S; CASTAÑÓN, A; CIANCIO N; ELESGARAY R

CABA

Congreso; III lnternational Congress in Translational; 2016

A moderate zinc deficient diet during pregnancy and/or growth induces low birth weight and renal dysfunction, such as reduced glomerular filtration rate, decreased activity of nitric oxide system, increased fibrotic processes and oxidative stress. These alterations are more evident in males than in females.The aim of this study was to evaluate the renal renin-angiotensin system (RAS) and systolic blood pressure (SBP) in adult male and female rats exposed to a moderate zinc deficient diet during fetal and/or postnatal growth.Female Wistar rats received during pregnancy up to weaning low (L:8ppm) or control (C:30ppm) zinc diet. After weaning L offspring, male(m) or female(f), fed low (l) or control (c) zinc diet during 60 days (Llm, Llf, Lcm, Lcf). C offspring fed control (c) zinc diet during their whole lives (Ccm, Ccf). At day 81 we measured SBP (mmHg) and we evaluated in renal cortex: AT-1R and AT-2R receptors by western blot (AT-1R or AT-2R/β-actin, DO relative to Ccm), Angiotensin-converting enzyme (ACE) mRNA expression by RT-qPCR (mRNA ACE/GAPDH relative to Ccm), and levels of Angiotensin-II (AngII) and Angiotensin-(1-7) (Ang1-7) by radioimmunoassay (nM/g tissue). Values are means±SEM, n=6/group. Two way ANOVA, Bonferroni posttest. *p