ESTABILIDAD DE UN EXTRACTO DE LARREA DIVARICATA CAV. SOMETIDO A DIGESTION SIMULADA

DEMIAN MONTI; MARIA ROSARIO ALOSNSO; IGNACIO PERALTA; CLAUDIA ANESINI

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Congreso; XLVIII Reunión Anual de a Sociedad Argentina de Farmacología Experimental (SAFE).; 2016

Sociedad Argentina de Farmacología Experimental

Medicinal plants are used as therapeutic agents butlittle is known about their pharmacokinetic properties especially the stabilityin gastric and intestinal fluids, phenomenon that affects theirbioavailability. Solubility and stability in gastrointestinalfluids  is normally a prerequisite forthe potential in vivo beneficial role of an extract given by an oral route. LarreadivaricataCav.is a South American plant widely distributed in Argentina, which aqueousextract exerts antioxidant, antitumoral and antimicrobial activities. Nevertheless,nothing is known about its stability in simulated digestion fluids. So the aimof this work was to study the stability but also the solubility of alyophilized aqueous extract of this plant compressed as a pill. In order to dothis, quantification of its majority polyphenol compound nor-dihydroguaiareticacid (NDGA), of the total polyphenols and flavonoids as well as the antioxidantactivity parameters such as  DPPHscavenger activity and reducing power were assayed after submitting the extractto different incubations time in simulated digestive fluids.The percentage of recovery of NDGA andtotal polyphenols was high, in the order of 90% in all fluids. Totalflavonoids slightly decreased after incubation in gastric fluid (60 min and 120min) from 12 to 18 % respectively. On the contrary incubations in intestinalfluid during 120 and 240 min provoked a higher decrease than in gastric fluidsreaching a value of 45 % and 40 % respectively. The DPPH scavenger activity as well as the reducingpower decreased in intestinal fluids 12% or 14 % respectively.These results suggested that NDGA did not change itssolubility depending on pH but also it didn?t suffer any metabolism in presenceof enzymes or pH variance. Polyphenols, such as NDGA, could balance out theactivity in view of flavonoids drop. We concluded that theextract was stable in gastrointestinal simulated fluids maintaining itsantioxidant activities which could support its use by an oral route.