Impact of Moderate Zinc Deficiency During Fetal Life and Postnatal Growth on Cardiac Nitric Oxyde System, Inflammatory and Oxidative Markers in Adult Male Rats

LORENA V JURIOL; NATALIA GOBETTO; FACUNDO MENDEZ GARRIDO; GONZALO PINEDA; MARINA DASSO; JORGE TOBLLI; ANDREA CARRANZA; ROSANA ELESGARAY; ANALÍA TOMAT; CRISTINA ARRANZ

Boston

Congreso; Experimental Biology 2015; 2015

Moderate zinc deficiency during fetal and postnatal growth programs cardiovascular dysfunction and hypertension. Male zinc deficient rats showed reduced left ventricular (LV) wall thickness and ejection fraction. Aim: to evaluate nitric oxide (NO) system, pro-inflammatory cytokines and oxidative state in LV of adult male rats exposed to this deficiency. Wistar rats received during pregnancy up to weaning low (L:8 ppm) or control (C:30 ppm) zinc diet. After weaning, male offspring fed low (l) or control (c) zinc diet during 60 days (Cc, Ll, Lc). At day 81, we evaluated in LV: Interleukin-6 and Tumor Necrosis Factor-α (IL-6;TNF-α), basal NO synthase (NOS) and endothelial, neuronal and inducible (eNOS;nNOS;iNOS) isoforms activities; eNOS and Ser1177 eNOS phosphorylation protein expression (eNOS/β-actin; pSer1177eNOS/eNOS), mRNA expression (eNOS/GAPDH) and lipid peroxidation end products (TBARS). Means±SEM, n=6/group. One way ANOVA, Bonferroni post-test. TNF-α (immunohistochemistry;% of positive staining/area): Cc:1.4±0.4; Ll:20.4±2.0*; Lc:1.7±0.3. IL-6 (immunohistochemistry; % of positive staining/area): Cc: 1.8±0.3; Ll: 21.1±1.7*; Lc: 2.8±0.8. Basal NOS Activity (pmol14C L-citrulline/g tissue.min): Cc: 204±6; Ll: 164±10*; Lc: 157±11*. Basal NOS+iNOS inhibitor (Aminoguanidine; pmol14C L-citrulline/g tissue.min): Cc: 216±7; Ll: 166±8*; Lc: 163±9*. Basal NOS+nNOS inhibitor (7-nitroindazole; pmol14C L-citrulline/g tissue.min) : Cc: 209±10; Ll: 166±8*; Lc: 164±9*. Basal NOS+Ca2+-calmodulin inhibitor (Calmidazolium; pmol14C L-citrulline/g tissue.min): Cc: 47±2#; Ll:52±2#; Lc:49±2#. pSer1177eNOS/eNOS (Western Blot; optical density relative to Cc): Cc:1.00±0.02; Ll:0.74±0.03*; Lc:1.02±0.16. TBARS (nmol/mg protein): Cc:0.21±0.02; Ll:0.78±0.08*; Lc:0.47±0.02*?. No differences were found in eNOS protein and mRNA expression. *p