IQUIMEFA 05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
In vivobiocompatibility evaluation of gentamicin silica-collagen nanocomposites
Autor/es:
MEBERT ANDREA MATHILDE; ALVAREZ GISELA; HELARY CHRISTOPHE; CORADIN THIBAUD; DESIMONE MARTÍN
Reunión:
Congreso; LI Reunión SAIB 2015; 2015
Resumen:
Nanocompositesarecompositesinwhichatleastoneofthephasesshowsnanometricdimensions.InthisworkweevaluatedinvivobiocompatibilityofSilicananoparticle-collagenIhydrogelscompositesasgentamicinreleasingsystemsformedicalapplications,thatshowedprolongedantibacterialactivityinapreviouswork.Onesteploaded500nmsilicananoparticles(SiNPs)weresynthesizedaccordingtotheStöbermethod.ForthispurposeTetraethylorthosilicate(TEOS)andammoniumhydroxidewereaddedtoawater-ethanolmixturewithorwithout(blank)gentamicinsulphate.MonodispersedrugfreeandgentamicinloadedSiNPswereobservedbyscanningelectronmicroscopy(SEM).CollagentypeIwaspurifiedfromrattailsandnanocompositeswerepreparedbymixing30mgSiNPswith5mg/mLcollagensuspension(0,5MSi).AntibacterialactivityofgentamicinSiNPs(338IUpergNPs)wereevaluatedbythediskdiffusionmethodasdescribedintheUSPharmacopeia.PseudomonasaeruginosawasusedassensitivemicroorganismandLBagarasgrowthmedium.CollagenhydrogelandnanocompositesmorphologywerealsoobservedbySEM.Biocompatibilitywasevaluatedinvivobyimplantingsubcutaneously300uLhydrogelorcompositeonWistarrats(250g).Nocleartoxicitywasobservedbyhistologyandimmunohistologicalstudiesafter15days.
