Study of the mechanism of the antiproliferative action of limonene, isolated from Tilia x viridis, upon a lymphoma cell line: participation of nitric oxide.

MANUELE, M G; BARREIRO ARCOS M L; FERRARO G; CREMASCHI G AND ANESINI C

BUENOS AIRES, ARGENTINA

Congreso; XXXVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE); 2007

Sociedad Argentina de Farmacología Experimental

The monoterpenes are not nutricional dietary compounds present in essential oil of vegetable species. In previous studies, we demonstrated that limonene, exerts antiproliferative action, upon a murine lymphoma cell line (BW 5147). Nitric oxide has been implicated in control of cell proliferation. The aim of the present work was to analyze the participation of nitric oxide in the antiproliferative action of limonene, isolated from Tilia x viridis, analyzing the participation of total nitrites specially NO in cell cycle and apoptosis, by flow citometry. Results (media ± SEM): proliferation on tumoral cells dpm (24 hs) Basal 22315± 1500; L10µg/ml: 19000 ± 1800; L10µg/ml+ L-NAME: 22800 ± 1900. Total nitrites 4 hs SI: L10µg/ml:  12,7 ± 1,5; L20 µg/ml: 16 ± 1,2 L 40 µg/ml: 8 ± 0,5. Cell cycle: % of cells in G0/G1 (4 hs): basal: 10.68 ± 1.5; L 10 µg/ml: 23.7 ± 2.0; L20 µg/ml: 20.35 ± 2.2. % of cells in G2/M (4 hs): basal: 32,93 ± 2,5; L10µg/ml: 23,7 ± 2,0; L10 µg/ml + L-NAME: 34,24 ± 2,5. % of subdiploid cells: basal: 15 ± 1.5; L10µg/ml: 25 ± 2,0;  L10µg/ml + L-NAME: 18 ± 1,2. The limonene presented antiproliferative action on lymphoma cell line, inducing cell arrest in G0/G1 and apoptosis  The nitric oxide is an intracellular signal  involved in these actions.