INFLUENCE OF NITRIC OXIDE SYSTEM AND OXIDATIVE STRESS IN THE CARDIAC ALTERATIONS INDUCED BY ZINC DEFICIENCY

ARRANZ CRISTINA; JURIOL LORENA VANESA; GOBETTO MARIA NATALIA; MENDES GARRIDO FACUNDO; CARDELLI ALCALDE DANIELA; BRUNELLO FRANCO; RADIONOVAS VANESA; ELESGARAY ROSANA; TOMAT ANALÍA LORENA

Atenas

Congreso; 24th European Meeting on Hypertension; 2014

European Society of Hypertension

Moderate zinc deficiency during intrauterine and postnatal growth induces cardiovascular disorders in adult males, characterized by an increase in blood pressure levels and a decrease in wall thickness and left ventricular (LV) contractility. In turn, coronary arteries exhibited a hypertrophic remodeling associated with increased blood pressure. Objective: To evaluate nitric oxide (NO) system and oxidative stress levels in adult male rats exposed to fetal and postnatal zinc deficiency. Female Wistar rats received during pregnancy up to weaning low (L:8 ppm) or control (C:30 ppm) zinc diet. After weaning, male offspring fed low (l) or control (c) zinc diet during 60 days (Cc, Ll, Lc). At day 81, we measured systolic blood pressure (SBP,mmHg,tail-cuff technique) and we evaluated in LV: basal NOS and endothelial(eNOS), neuronal(nNOS) e inducible(iNOS) isoforms activities (pmol 14C L-citrulline/g.tissue.min); eNOS protein expression (western blot; optical density eNOS/β-actin relative to Cc) and mRNA expression (RT-qPCR; eNOS/GAPDH relative to Cc), lipid peroxidation end products (TBARS, nmol/mg protein) levels, catalase(pmol/mg protein), glutathione peroxidase(GPx, µmol/min.mg.protein), superoxide dismutase(SOD, USOD/mg protein) activities and glutathione concentration(GLUT, mg/mg protein). Values are means±SEM, n=6/group. One way ANOVA, Bonferroni post-test Results *p