Influence of atrial natriuretic peptide treatment in vascular response in male and female hypertensive rats.

COSTA A.; ROMERO M; BOUCHET G; CANIFFI C.; SAVIGNANO L; GOBETTO N; JURIOL L; ELESGARAY R.; MC LAUGHLIN M; ARRANZ C.

Londres

Congreso; Twenty-fourth European Meeting on Hypertension.; 2012

European Society of hypertension

The aim was to investigate vascular effects of chronic infusion with atrial natriuretic peptide (ANP) in spontaneously hypertensive rats (SHR), and analyze differences between gender. Methods: 10 weeks-old male (M) and female (F) SHR were infused (14 days, osmotic pumps) with ANP (100 ng/hr rat) or saline (S).Systolic blood pressure (SBP, mmHg) and nitrites and nitrates excretion (NOx, nmol/min·100 g) were determined. Animals were sacrificed to perform morphological studies in thoracic aorta sections stained with hematoxylin-eosin (media/lumen ratio, ìm/mm) and fibrosis signs with Sirius Red (ASR) (Score). Aortic NOS activity 14C L-arginine, pmol/g·tissue·min) was determined. Cardiac ventricle and kidney sections were stained with Sirius Red, to evaluate perivascular collagen in coronary (CA) and renal arteries (RA) (ìm/mm lumen). osmotic pumps) with ANP (100 ng/hr rat) or saline (S).Systolic blood pressure (SBP, mmHg) and nitrites and nitrates excretion (NOx, nmol/min·100 g) were determined. Animals were sacrificed to perform morphological studies in thoracic aorta sections stained with hematoxylin-eosin (media/lumen ratio, ìm/mm) and fibrosis signs with Sirius Red (ASR) (Score). Aortic NOS activity 14C L-arginine, pmol/g·tissue·min) was determined. Cardiac ventricle and kidney sections were stained with Sirius Red, to evaluate perivascular collagen in coronary (CA) and renal arteries (RA) (ìm/mm lumen). 10 weeks-old male (M) and female (F) SHR were infused (14 days, osmotic pumps) with ANP (100 ng/hr rat) or saline (S).Systolic blood pressure (SBP, mmHg) and nitrites and nitrates excretion (NOx, nmol/min·100 g) were determined. Animals were sacrificed to perform morphological studies in thoracic aorta sections stained with hematoxylin-eosin (media/lumen ratio, ìm/mm) and fibrosis signs with Sirius Red (ASR) (Score). Aortic NOS activity 14C L-arginine, pmol/g·tissue·min) was determined. Cardiac ventricle and kidney sections were stained with Sirius Red, to evaluate perivascular collagen in coronary (CA) and renal arteries (RA) (ìm/mm lumen). Results: In coronary and renal arteries, perivascular collagen area was higher in M than in F SHR and ANP reduced this parameter in both sex. M than in F SHR and ANP reduced this parameter in both sex. In coronary and renal arteries, perivascular collagen area was higher in M than in F SHR and ANP reduced this parameter in both sex. Conclusion: ANP treatment increased vascular NO system, improving aortic wall properties and modifying coronary and renal arteries remodeling in SHR wall properties and modifying coronary and renal arteries remodeling in SHR ANP treatment increased vascular NO system, improving aortic wall properties and modifying coronary and renal arteries remodeling in SHR