NEUTRALIZACIÓN DE EXOPRODUCTOS DE PSEUDOMONAS AERUGINOSA POR ANTICUERPOS GENERADOS CONTRA LARREA DIVARICATA CAV. (jarilla)

SASSO CV.; MATTAR MA; DAVICINO R; MARTINO R, ; MICALIZZI B

Mendoza

Congreso; XXVIII Reunión Anual Científica de la Sociedad de Biología de Cuyo.; 2010

Sociedad de Biología de Cuyo

Larrea divaricata Cav. (jarilla) is widely used in Argentina in folk therapy to treat of a number of conditions, but little is known about its immunological properties. Pseudomonas aeruginosa is a Gram negative bacilli considered a nosocomial pathogen. The aim of this study was to evaluate the cross reaction between proteins from crude extract of jarilla (JPCE) and extracellular bacterial proteins (EP), which exhibit enzymatic and cytotoxic activity. JPCE was obtained from leaves in PBS pH 7.4 for 24 h at 4oC and concentrated by ultrafiltration. The strain ATCC 27853 of P. aeruginosa was used. The EP were obtained from the supernatant, dialyzed, and lyophilized. Assays of hemolytic and proteolytic activity were carried out. The effects of EP on macrophage viability, apoptosis and phagocitosis were tested. The anti-hemolytic and anti-proteolytic activities of an anti-JPCE serum were assayed by incubation for 1h at 37°C. Both, hemolytic and proteolytic activities were inhibited (55-95%) by anti-JPCE serum. Cell viability and phagocitosis were increased after incubation of EP with anti-JPCE serum. The results obtained showed that proteins from L. divaricata Cav. are able to generate antibodies that cross-react with EP from P. aeruginosa. These results could be relevant in the development of alternative therapies (i.e. vaccines) against infections produced by P. aeruginosa.Cav. (jarilla) is widely used in Argentina in folk therapy to treat of a number of conditions, but little is known about its immunological properties. Pseudomonas aeruginosa is a Gram negative bacilli considered a nosocomial pathogen. The aim of this study was to evaluate the cross reaction between proteins from crude extract of jarilla (JPCE) and extracellular bacterial proteins (EP), which exhibit enzymatic and cytotoxic activity. JPCE was obtained from leaves in PBS pH 7.4 for 24 h at 4oC and concentrated by ultrafiltration. The strain ATCC 27853 of P. aeruginosa was used. The EP were obtained from the supernatant, dialyzed, and lyophilized. Assays of hemolytic and proteolytic activity were carried out. The effects of EP on macrophage viability, apoptosis and phagocitosis were tested. The anti-hemolytic and anti-proteolytic activities of an anti-JPCE serum were assayed by incubation for 1h at 37°C. Both, hemolytic and proteolytic activities were inhibited (55-95%) by anti-JPCE serum. Cell viability and phagocitosis were increased after incubation of EP with anti-JPCE serum. The results obtained showed that proteins from L. divaricata Cav. are able to generate antibodies that cross-react with EP from P. aeruginosa. These results could be relevant in the development of alternative therapies (i.e. vaccines) against infections produced by P. aeruginosa.Pseudomonas aeruginosa is a Gram negative bacilli considered a nosocomial pathogen. The aim of this study was to evaluate the cross reaction between proteins from crude extract of jarilla (JPCE) and extracellular bacterial proteins (EP), which exhibit enzymatic and cytotoxic activity. JPCE was obtained from leaves in PBS pH 7.4 for 24 h at 4oC and concentrated by ultrafiltration. The strain ATCC 27853 of P. aeruginosa was used. The EP were obtained from the supernatant, dialyzed, and lyophilized. Assays of hemolytic and proteolytic activity were carried out. The effects of EP on macrophage viability, apoptosis and phagocitosis were tested. The anti-hemolytic and anti-proteolytic activities of an anti-JPCE serum were assayed by incubation for 1h at 37°C. Both, hemolytic and proteolytic activities were inhibited (55-95%) by anti-JPCE serum. Cell viability and phagocitosis were increased after incubation of EP with anti-JPCE serum. The results obtained showed that proteins from L. divaricata Cav. are able to generate antibodies that cross-react with EP from P. aeruginosa. These results could be relevant in the development of alternative therapies (i.e. vaccines) against infections produced by P. aeruginosa.P. aeruginosa was used. The EP were obtained from the supernatant, dialyzed, and lyophilized. Assays of hemolytic and proteolytic activity were carried out. The effects of EP on macrophage viability, apoptosis and phagocitosis were tested. The anti-hemolytic and anti-proteolytic activities of an anti-JPCE serum were assayed by incubation for 1h at 37°C. Both, hemolytic and proteolytic activities were inhibited (55-95%) by anti-JPCE serum. Cell viability and phagocitosis were increased after incubation of EP with anti-JPCE serum. The results obtained showed that proteins from L. divaricata Cav. are able to generate antibodies that cross-react with EP from P. aeruginosa. These results could be relevant in the development of alternative therapies (i.e. vaccines) against infections produced by P. aeruginosa.was used. The EP were obtained from the supernatant, dialyzed, and lyophilized. Assays of hemolytic and proteolytic activity were carried out. The effects of EP on macrophage viability, apoptosis and phagocitosis were tested. The anti-hemolytic and anti-proteolytic activities of an anti-JPCE serum were assayed by incubation for 1h at 37°C. Both, hemolytic and proteolytic activities were inhibited (55-95%) by anti-JPCE serum. Cell viability and phagocitosis were increased after incubation of EP with anti-JPCE serum. The results obtained showed that proteins from L. divaricata Cav. are able to generate antibodies that cross-react with EP from P. aeruginosa. These results could be relevant in the development of alternative therapies (i.e. vaccines) against infections produced by P. aeruginosa.°C. Both, hemolytic and proteolytic activities were inhibited (55-95%) by anti-JPCE serum. Cell viability and phagocitosis were increased after incubation of EP with anti-JPCE serum. The results obtained showed that proteins from L. divaricata Cav. are able to generate antibodies that cross-react with EP from P. aeruginosa. These results could be relevant in the development of alternative therapies (i.e. vaccines) against infections produced by P. aeruginosa.L. divaricata Cav. are able to generate antibodies that cross-react with EP from P. aeruginosa. These results could be relevant in the development of alternative therapies (i.e. vaccines) against infections produced by P. aeruginosa.P. aeruginosa. These results could be relevant in the development of alternative therapies (i.e. vaccines) against infections produced by P. aeruginosa.P. aeruginosa.