IQUIMEFA   05518
INSTITUTO QUIMICA Y METABOLISMO DEL FARMACO
Unidad Ejecutora - UE
artículos
Título:
Infrared Spectroscopic Analysis of Urinary Calculi: A Retrospective Study in Argentinean Patients.
Autor/es:
MUSCHIETTI L.; GARRIDO G.; CAMPO DALL´ ORTO V.
Revista:
Asian Journal of Medicine and Health
Editorial:
SCIENCEDOMAIN international
Referencias:
Año: 2016 vol. 1 p. 1 - 9
Resumen:
Aims: To investigate, by infrared spectroscopy (FT-IR), the chemical composition of urinarycalculi obtained from patients of Buenos Aires, Argentina.Duration of Study:The composition of the urinary calculi was evaluated in a retrospectivestudy from March 1993 to September 2013.Methodology:Infrared spectra of the urinary calculi were recorded in a Bruker IFS-25 FT-IRand in a Nicolet 380 FT-IR spectrophotometers. We included 440 urinary stones (286 frommen and 154 from women). The samples were obtained by spontaneous passage,shockwave lithotripsy, ureteroscopy or percutaneous nephrolithotripsy mainly from patientstreated at Hospital de Clínicas, University of Buenos Aires.Results:Calcium oxalate (both in pure or mixed samples) was detected in 326 cases (74.09%). Anhydrous uric acid (8.41 %) was observed most frequently, followed by struvite (2.05%) and cystine (2.50 %). For some chemical compounds, a significant gender-relateddifference was found. Applying the second derivative spectra allowed to distinguish betweenthe presence of whewellite, weddellite and their mixture. More than 70% of recurrent urinarystones were of the same chemical composition.Conclusion:FT-IR analysis of urinary calculi over a period of 20 years gave an outlook ofthe prevalence of certain stone components in patients from Buenos Aires, Argentina, whichin some cases were found to be gender-related. The results obtained are in accordance withstatistics from other industrialized countries, except for uric acid (15.93%), even pure orcombined in other forms, which was more frequent than the world prevalence (up to 10%).FT-IR spectroscopy combined with the second derivative method of analysis proved to be apowerful tool to discriminate mixed oxalates whose composition only differed in one watermolecule.