Effect of Eupatorium arnottianum on Gastrointestinal Tract

S.GORZALCZANY ; M. CLAVIN ; V. MARTINO; P. CAMINOS

European Journal of Medicinal Plants

Sciencedomain International

Lugar: Nashik; Año: 2016 vol. 15 p. 1 - 7

2231-0894

Aims: Eupatorium arnottianum Griseb is used in popular medicine for gastric pains. So, the aim ofthis study was to investigate the possible spasmolytic effects of ethanolic and dichloromethanicextracts of the plant using preclinical methods.Place and Duration of Study: Department of Pharmacology, School of Pharmacy andBiochemistry, Universidad de Buenos Aires, from September 2015 to May 2016.Methodology: The effects of Eupatorium arnottianum extracts and its isolated compounds wereevaluated on isolated rat jejunum. Intestinal transit of charcoal meal after the administration ofextract was determined and compared with the control group. Also, phytochemical study wasperformed.Results: The extracts inhibited non competitively the cumulative concentration?response curvesinduced by acetylcholine (1.10-9?1.10-5 M), with similar inhibition at the highest concentration (2mg/mL: 66.2±8.7% of inhibition for ethanolic extract and 61.5±10.5% of inhibition forOriginal Research ArticleGorzalczany et al.; EJMP, 15(4): 1-7, 2016; Article no.EJMP.280092dichlorometanic extract). Also, both extracts non-competitively inhibited the curve responseconcentration induced by CaCl2 (1.10-4 -3. 10-1 M) and significantly reduced the maximal responsein a concentration-dependent manner. Furthermore, E. arnottianum (62, 125 and 250 mg/Kg, peroral) significantly decreased the propulsion of the charcoal meal through the gastrointestinal tract(68% of inhibition for 250 mg/Kg ethanolic extract, 42% of inhibition for 125 mg/Kg dichlorometanicextract). Finally, nepetin, the major compound of the ethanolic extract and two major compounds ofdichlorometanic extract, jaceosidin and nepetin, reduced the maximal response of theacethylcholine in isolated jejunum (30 μg/mL: 75% of inhibition for jaceosidin and 89% of inhibitionfor nepetin).Conclusion: The results of the present study could demonstrate that Eupatorium arnottianumplays a spasmolytic role in gastrointestinal motility by interfering in calcium influx. Also nepetin andjaceosidin, major components of ethanolic and dichlorometanic extracts could be responsible forthese properties.